QUIZ – TransBio

Ventilator‑induced lung injury models

Correct answer: B

Explanation: The framework of the 3Rs guides ethical and scientifically rigorous animal research in translational medicine:

• Replacement (use non-animal methods when possible (organoids, in-silico models, microfluidics))
• Reduction (obtain the same scientific information using fewer animals)
• Refinement (minimize suffering and improve welfare)

A fourth R, hence “4R” such as Reproducibility (ensure robust, translatable experimental design) is often added. In this scenario, longitudinal imaging allows multiple measurements in the same animal, meaning fewer animals are required to achieve adequate statistical power.

Therefore, the primary principle applied is Reduction.